TECHNIQUES FOR OBTAINING SMEAR AND CULTURE SPECIMENS
Endocervical canal
A vaginal speculum should be used to visualise the cervix. The speculum should be moistened with warm water, do not use any other lubricant.
Remove excess cervical mucus, preferably with a cotton ball held in ring forceps.
Insert a sterile cotton-tipped applicator into the cervical canal; rotate swab; allow 10 to 30 seconds for absorption of organisms on to the swab.
Use a sterile cotton-tipped applicator to obtain swabs of the lateral wall of the vagina or the posterior fornix.
Urethra
Wipe away frank pus and exudate. Use a sterile bacteriological loop or a disposable plastic loop or a sterile urethral swab moistened with sterile saline.
The loop or swab is inserted several centimetres to reach the columnar epithelium of the anterior urethra which is scraped.
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